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Application Data

- Measurement of Food Allergens with Microplate Reader -

2008.10.1 Corona Electric.Co., Ltd

In recent years, consumers have become increasingly aware of food safety. June 2008, Health, Labour and Welfare Ministry decided that food containing ingredient which derive from prawn and crab are to be mandatory labeled as well as other 5 kinds of ingredient. Detection of food allergen based on official method, ELISA method, require microplate reader system. Microplate system from Corona includes data treatment software supporting standard curve routine of 4-coefficient logistic function. The Microplate system will provide you with easy-to-follow operation flow from measurement to report formatting.

Dilute crushed sample(20-fold)   Shaking (12 hours or more) Centrifugation (3,000G, 20 minutes)   Keep in room ambient (20-25°C,1hour) Dispense standard and test article(100µL/well)   Keep in room ambient (20-25°C,1hour) Rinsing (300µL / well, 5 times) Add enzyme-labeled antibody (100µL/well)   Keep in room ambient (20-25°C,1hour) Add enzyme-labeled antibody (100µL/well) Add enzyme substrate (100µL / well)   Keep in room ambient       (at light-shielded place20-25°C, 1hour) Add quencher (100µL/well) Absorption Measurements (Wavelength: 450nm, Reference: 650nm) Result Sample Judgment Figure(µg/g) Praun Rice Snack Positive 20 < Instant Noodle Positive 20 < Potato Chip Negative < 1.0

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