Application Data (Human Cytochrome P450SNP)

Application Data

- Measurement with Syber^® Green Ι, Typing kit for Human Cytochrome P450SNP -

Feature
Detection of genetic polymorphism based on ASP-PCR method enables prompt and high-throughput detection using fluorescence microplate reader.

* ASP : Allele Specific Primer

+ Syber ^® Green I spends only 3 hours !
for measurement that electrocataphoresis spends full a day.

Test Flow

Collection of sample

Extraction of DNA

Adjustment of Conc.

ASP-PCR

← 100µL of Syber® Green I
shall be dispensed on plate.

FL measurement with a microplate reader

Reagent

SNP Typing Kit
Cytochrome P450 2D6*10(100C→T)

¥ 40,000 / 200 times

Toyobo Co., Ltd.

Measuring Instrument

MTP-600F (Filter : EX 360nm, EM 535nm)

MTP-600F	¥ 3,500,000
EM 535 Filter	¥ 46,000

Setting Condition
MODE1	:	FL intensity
MODE2	:	1Ex-1Em
Ex	:	360
Em	:	535
SENS	:	×10
Direction	:	Vertical
RESPONSE	:	4
MIXINGTIME	:	20S
MIXINGMODE	:	Linear
MIXINGLEVEL	:	Middle
TEMP	:	OFF
KINETIC	:	OFF

Result

CV value is among 96 wells are less then CV10%.

5 times of repeating measurement with typing kit on MTP-600F.(CV value) Unit:%

Result of Measurement

This archive is based on Instruction by Dr.Kubota at Tokyo University Hospital.

Syber^® Green is registered brand of Molecular Probes, Inc.